【Abstract】 Objective To study the anti?fertility effect of gossypol to Lasiopodomys brandtii. Methods The effect of gossypol to sperm quality, testis and epididymis tissues of L.brandtii was tested with one?off quantitative gastric gavages. Results Gossypol did harm to spermatids, spermatocytes and testis tissue lesions. Testis and epididymis tissues coefficient decreased obviously at the dosage of 20-100 mg/kg, and sperm concentration reduced and sperm malformation increased at 20 mg/kg. It leaded to the death of L.brandtii when the dosage exceeded 60 mg/kg. Conclusion The gossypol had significant anti?fertility effect on male L.brandtii at the concentration of 20-100 mg/kg, but it was lethal at high concentrtion.